|
KMID : 0357119790010010025
|
|
Korean Journal of Immunology
1979 Volume.1 No. 1 p.25 ~ p.33
|
|
|
Indirect Hemagglutination Test with Antigens Extracted from Propionibacterium acnes
|
|
梁容泰/Yang, Yong Tae
任春彬/崔哲淳/Im, Choon Bin/Choi, Chul Soon
|
|
|
Abstract
|
|
|
Propionibacterium acnes, rather well known as Corynebacterium parvum in the fields of experimental and clinical medicine, is a member of the Gram-positive, non-sporeforming, anaerobic bacilli.
Since the sensitivity and specificity of indirect hemagglutination is well established, it appeared quite plausible that the development of indirect hemagglutination test with the antigens extracted from P. acnes would sufficiently facilitate the immunological studies on P. acnes such as the classification of P. acnes into type 1 and type 11, determination of the levels of antibodies against P. acnes in the sera of healthy or diseased individuals, and the nature of host-parasite interactions between human and P. acnes as a whole.
The purpose of this study included 1) extraction of hemagglutinating antigens from P. acnes, 2) standardization of indirect hemagglutination test with the extracted antigens, and 3) comparative measurements of antibody titers in the sera of hyperimmunized rabbits, and apparently healthy individuals against P. acnes by agglutination and indirect hemagglutination test.
This study clearly demonstrated that the hemagglutinating antigens could be extracted from P. acnes and the sheep red blood cells sensitized with those antigens agglutinated in the presence of specific antibodies.
The results are summarized as follows.
1. Of the three hemagglutinating antigens of P. acnes the antigen 3, extracted by the method of Chun et al. (1957) provided the best sensitization of sheep red blood cells.
2. Among the normal, formalinized and tanned sheep red blood cells, the use of the forrralinized one appeared most appropriate for the storage and the sensitivity in the hemagglutination test.
3. Indirect hemagglutination test was more sensitive than agar gel diffusion.
4. Hemagglutinating antibody content against P. acnes in the hyperimmune rabbits sera were much lower than the agglutinin contents,__ and the hemagglutination tests with homologous P. acnes type sera failed to show type specificity of the reaction.
5. The result of comparative titration of agglutinin and hemagglutinating antibody against P. acnes
type I in the sera of 34 apparently healthy individuals also showed that the hemagglutinating
antibody titers were much lower than the agglutinin titers, and that the majority of hemagglutinating
antibody titers of the sera clustered in the levels of lower titers than those of agglutinin.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|